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Cross-sectional staining A fully differentiated intestinal monolayer was used for immunofluorescence imaging of functional enterocyte markers (FABP1 and ZO-1, 1:100)

Woth G et al (2014) The effect of Na-selenite treatment on the oxidative stressantioxidants balance of multiple organ failure

After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]

The process of reconstitution, turning that stable powder back into a usable liquid solution, is far more than just adding water

This can be mistaken for feeling generally cold